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Related Experiment Videos

Murine Hoxc-9 gene contains a structurally and functionally conserved enhancer

T Papenbrock1, R L Peterson, R S Lee

  • 1Department of Medicine, Medical University of South Carolina, Charleston 29425, USA.

Developmental Dynamics : an Official Publication of the American Association of Anatomists
|August 26, 1998
PubMed
Summary

Researchers identified a conserved enhancer within the Hoxc-9 gene that regulates gene expression in the developing nervous system. This enhancer functions similarly in mice, chickens, and flies, highlighting ancient regulatory mechanisms.

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Area of Science:

  • Developmental Biology
  • Genetics
  • Evolutionary Biology

Background:

  • The Hoxc-9 gene plays a crucial role in embryonic development, particularly in the posterior neural tube.
  • Understanding gene regulation is key to deciphering developmental processes and evolutionary conservation.

Purpose of the Study:

  • To identify and characterize regulatory elements within the Hoxc-9 genomic region.
  • To investigate the functional conservation of the Hoxc-9 enhancer across different species.

Main Methods:

  • Reporter gene analysis in transgenic mice and Drosophila.
  • Sequence comparison between mouse and chicken Hoxc-9 introns.
  • Deletion analysis of conserved sequence elements (CSEs).

Main Results:

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  • A positional enhancer was identified in the mouse Hoxc-9 intron, driving posterior neural tube expression.
  • Two highly conserved sequence elements (CSEs) were found in mouse and chicken Hoxc-9 introns, essential for enhancer activity.
  • The mouse Hoxc-9 enhancer demonstrated conserved function in Drosophila, directing posterior gene expression in the central nervous system.

Conclusions:

  • The Hoxc-9 intragenic enhancer exhibits conserved function across vertebrates and arthropods.
  • Cooperation between CSEs is crucial for the full activity of the Hoxc-9 enhancer.
  • This study reveals ancient transcriptional regulatory circuits conserved from invertebrates to vertebrates.