Bone formation via cartilage models: the "borderline" chondrocyte

Increasing evidence substantiates the view that death is not necessarily the only fate of hypertrophic chondrocytes and that, when exposed to the right microenvironment, these cells can further differentiate to osteoblast-like cells and contribute to initial bone formation. In vitro, when replated as adherent cells in the presence of ascorbic acid, hypertrophic chondrocytes resume cell proliferation, switch from the synthesis of the cartilage-characteristic type II and X collagens to the synthesis of type I collagen, and organize a mineralizing bone matrix. In vivo, expression of bone specific markers by growth plate chondrocytes occurs initially in early hypertrophic cells located at the mid-diaphysis and directly facing the osteogenic perichondrium. In bones formed via cartilage models, the first mineralized bone matrix (the earliest bony collar preceding vascular invasion and the onset of endochondral bone formation) is deposited at the outer aspect of the mid-diaphysis between rows of early hypertrophic chondrocytes and osteoblasts, which are arranged in a peculiar "vis à vis" fashion. The "vis à vis" organization of perichondrial osteogenic cells and peripheral early hypertrophic chondrocytes suggests that the latter cells are exposed -- compared to their cognate, the central hypertrophic chondrocytes -- to a specific microenvironment composed of unique matrix-originating signals and cellular cross-talks. A major role in the differentiation control of, and interaction between, hypertrophic chondrocytes and osteogenic perichondrial cells is certainly played by the Indian Hedgehog/PTHrP signalling system. We propose that all early hypertrophic chondrocytes have the inherent potential to differentiate to osteoblast-like cells and to contribute to initial bone formation, but that only chondrocytes positioned at the "borderland" between cartilage and (non-cartilage) osteogenic tissues undergo further differentiation to bone producing cells. We call these hypertrophic chondrocytes "borderline chondrocytes" to emphasize both their specific location and their dual differentiation potential. Hypertrophic chondrocytes located in different cartilage areas are exposed to an inappropriate matrix and endocrine/paracrine environment, cannot differentiate to osteoblast-like cells and therefore undergo apoptosis.