Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Inositol phospholipids: translocation, translocation, translocation

R Irvine1

  • 1Department of Pharmacology University of Cambridge Tennis Court Road, Cambridge CB2 1QJ, UK. rfi20@cam.ac.uk

Current Biology : CB
|August 26, 1998
PubMed
Summary

Pleckstrin homology (PH) domains bind to inositol phospholipids. These interactions are crucial for directing proteins within cells after changes in lipid levels.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The provision of surgical care for children with cardiac disease: the Jamaican experience--an 18-year review.

The West Indian medical journal·2012
Same author

Risk of significant gynaecological pathology in women with glandular neoplasia on cervical cytology.

Cytopathology : official journal of the British Society for Clinical Cytology·2011
Same author

Prevalence of hepatic lesions at post-mortem examination in dogs and association with pancreatitis.

The Journal of small animal practice·2010
Same author

[Salivary stones and stenosis. A comprehensive classification].

Revue de stomatologie et de chirurgie maxillo-faciale·2008
Same author

Salivary stones and stenosis. A comprehensive classification.

Revue de stomatologie et de chirurgie maxillo-faciale·2008
Same author

Vasopressin for refractory hypotension during cardiopulmonary bypass.

The West Indian medical journal·2008

Area of Science:

  • Cellular Biology
  • Molecular Biology
  • Biochemistry

Background:

  • Pleckstrin homology (PH) domains are protein modules known to bind inositol phospholipids.
  • The functional significance of these interactions in vivo is under investigation.

Purpose of the Study:

  • To explore the role of PH domain and inositol phospholipid interactions in protein translocation.
  • To understand how these interactions are regulated by stimulus-induced lipid metabolism.

Main Methods:

  • In vitro binding assays to study PH domain-phospholipid interactions.
  • Cellular experiments to track protein localization following lipid changes.

Main Results:

  • Confirmed in vitro interaction between PH domains and various inositol phospholipids.
  • Demonstrated that these interactions influence protein movement within the cell.
  • Observed correlation between lipid metabolism changes and protein translocation.

Conclusions:

  • PH domain-phospholipid interactions are critical for targeted protein delivery within cells.
  • These interactions serve as a mechanism for spatial regulation of proteins in response to cellular signals.

Related Experiment Videos