Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

ATM binds to beta-adaptin in cytoplasmic vesicles

D S Lim1, D G Kirsch, C E Canman

  • 1Oncology Center, The Johns Hopkins School of Medicine, Baltimore, MD 21205, USA.

Proceedings of the National Academy of Sciences of the United States of America
|August 26, 1998
PubMed
Summary

Ataxia telangiectasia mutated (ATM) protein interacts with beta-adaptin, a key component in cellular transport. This finding suggests ATM’s role in intracellular vesicle and protein transport mechanisms.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A randomized phase III trial of anthracyclines followed by taxane versus taxane plus carboplatin as (neo)adjuvant therapy in patients with triple-negative breast cancer: KCSG BR 15-1 PEARLY trial.

Annals of oncology : official journal of the European Society for Medical Oncology·2026
Same author

Performance of summary measures of core body temperature in heat-related health outcome regression models.

International journal of biometeorology·2025
Same author

Safety and efficacy of trastuzumab biosimilar plus irinotecan or gemcitabine in patients with previously treated HER2 (ERBB2)-positive non-breast/non-gastric solid tumors: a phase II basket trial with circulating tumor DNA analysis.

ESMO open·2023
Same author

Is there a clinical difference in paediatric congenital cholesteatoma according to age?

The Journal of laryngology and otology·2022
Same author

Early line-probe assay using DNA specimens in patients with pulmonary TB.

The international journal of tuberculosis and lung disease : the official journal of the International Union against Tuberculosis and Lung Disease·2022
Same author

Potential role of CMPK1, SLC29A1, and TLE4 polymorphisms in gemcitabine-based chemotherapy in HER2-negative metastatic breast cancer patients: pharmacogenetic study results from the prospective randomized phase II study of eribulin plus gemcitabine versus paclitaxel plus gemcitabine (KCSG-BR-13-11).

ESMO open·2021

Area of Science:

  • Molecular Biology
  • Cell Biology
  • Genetics

Background:

  • Inherited ATM gene mutations cause ataxia telangiectasia, a disorder with neurological, immunological, and cancer-related symptoms.
  • The ATM protein's precise cellular functions beyond DNA repair are not fully understood.

Purpose of the Study:

  • To investigate potential novel interactions of the ATM protein within cellular pathways.
  • To explore ATM's role in intracellular trafficking and protein transport.

Main Methods:

  • Yeast two-hybrid screening to identify ATM-interacting proteins.
  • In vitro binding assays to confirm interactions.
  • Coimmunoprecipitation and colocalization studies to validate in vivo association.

Main Results:

Related Experiment Videos

  • ATM directly binds to beta-adaptin, a component of the AP-2 clathrin-mediated endocytosis complex.
  • ATM also interacts with beta-NAP, a neuronal beta-adaptin homolog.
  • ATM and beta-adaptin co-localize within cellular vesicles, indicating a functional association.

Conclusions:

  • ATM associates with beta-adaptin and beta-NAP, suggesting a role in vesicle-mediated transport.
  • These findings implicate ATM in intracellular protein and vesicle trafficking pathways, potentially linking its function to neuronal degeneration observed in ataxia telangiectasia.