Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Consistent, persistent expression from modified retroviral vectors in murine hematopoietic stem cells

P B Robbins1, D C Skelton, X J Yu

  • 1Department of Molecular Microbiology and Immunology, Division of Research Immunology/Bone Marrow Transplantation, Childrens Hospital Los Angeles, University of Southern California School of Medicine, Los Angeles, CA 90027, USA.

Proceedings of the National Academy of Sciences of the United States of America
|August 26, 1998
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

[Progress in research of multi-omics on risk for schizophrenia onset].

Zhonghua liu xing bing xue za zhi = Zhonghua liuxingbingxue zazhi·2025
Same author

[Challenges and strategies in minimally invasive pancreatic enucleation].

Zhonghua wai ke za zhi [Chinese journal of surgery]·2025
Same author

[Interpretation of the surgical treatment of pancreatic cancer of the 2024 edition of the NCCN guidelines].

Zhonghua wai ke za zhi [Chinese journal of surgery]·2024
Same author

Corrigendum to "Mutations and clinical significance of calcium voltage-gated channel subunit alpha 1E (CACNA1E) in non-small cell lung cancer" [Cell Calcium 102 (2022) 102527].

Cell calcium·2024
Same author

[Research progress on the role of resveratrol in wound healing].

Zhonghua shao shang yu chuang mian xiu fu za zhi·2024
Same author

[Research progress on HEG1 in cardiovascular generation and tumor development].

Zhonghua yu fang yi xue za zhi [Chinese journal of preventive medicine]·2024

Modified retroviral vectors show significantly improved gene expression and reduced DNA methylation in hematopoietic stem cells compared to standard Moloney murine leukemia virus (MoMuLV)-based vectors in vivo.

Area of Science:

  • Gene therapy
  • Retroviral vector development
  • Hematopoietic stem cell biology

Background:

  • Moloney murine leukemia virus (MoMuLV)-based retroviral vectors exhibit variable gene expression and in vivo methylation.
  • Internal regulatory elements within MoMuLV vectors can impede long terminal repeat-mediated expression.
  • Previous in vitro studies demonstrated superior performance of modified retroviral vectors.

Purpose of the Study:

  • To evaluate the in vivo efficacy of modified retroviral vectors in hematopoietic stem cells.
  • To assess gene expression levels and proviral methylation in hematopoietic colonies derived from transduced bone marrow.
  • To compare the performance of modified vectors against standard MoMuLV-based vectors in a serial transplantation model.

Main Methods:

Related Experiment Videos

  • Serial transplantation of mouse bone marrow transduced with modified or standard MoMuLV-based retroviral vectors.
  • Analysis of gene expression in differentiated hematopoietic colonies (2 degreesCFU-S) within mouse spleens.
  • Assessment of proviral DNA methylation in vector-containing hematopoietic cells.

    • Main Results:

    • Modified vectors achieved significantly higher expression frequency (>90%) compared to standard MoMuLV vectors (28%) in vector-containing 2 degreesCFU-S.
    • Expression from modified vectors was consistent across recipients (>50% in all analyzed), unlike standard vectors which showed variable expression (0-50%).
    • Modified vectors exhibited lower levels of DNA methylation than control MoMuLV-based vectors.

    Conclusions:

    • Modified retroviral vectors offer superior and consistent gene expression in hematopoietic stem cells in vivo.
    • Reduced DNA methylation in modified vectors contributes to enhanced and sustained transgene expression.
    • These findings advance the development of effective retroviral vectors for in vivo gene therapy applications.