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Design and anti-HIV-1 activity of hammerhead and hairpin ribozymes containing a stable loop
1Exploratory Chemistry Research Laboratories, Sankyo Co., Ltd., Tokyo, Japan. koizum@shina.sankyo.co.jp
Nucleosides & Nucleotides
|August 26, 1998
Abstract:
Three ribozymes, a hairpin ribozyme (HR112) and two hammerhead ribozymes (RZ115 and RZ119) containing a 5'C(UUCG)G3' loop were designed to cleave the U5 region in the long terminal repeat (LTR) of HIV-1 RNA. The t1/2 values of chemically synthesized substrates mediated by three ribozymes were measured. The transformed CEM cells possessing these three ribozyme-encoding genes were challenged with a HIV-1IIIB strain, and two of these three ribozymes, HR112 and RZ119, were shown to possess strong anti-HIV-1 activity.