Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

The oxygen fixation hypothesis: a reevaluation

D Ewing1

  • 1Department of Radiation Oncology, Allegheny University of the Health Sciences, Hahnemann, Philadephia, Pennsylvania 19102, USA.

American Journal of Clinical Oncology
|August 26, 1998
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Synergistic neutralizing antibody response to a dengue virus type 2 DNA vaccine by incorporation of lysosome-associated membrane protein sequences and use of plasmid expressing GM-CSF.

Virology·2002
Same author

International Child Care Practices Study: infant sleeping environment.

Early human development·2001
Same author

Dengue virus type 1 DNA vaccine induces protective immune responses in rhesus macaques.

The Journal of general virology·2000
Same author

Immunogenicity of dengue virus type 1 DNA vaccines expressing truncated and full length envelope protein.

Vaccine·2000
Same author

Production of radiation-resistant E. coli strains by daily X-irradiation.

International journal of radiation biology·1997
Same author

Genetic, antigenic and serologic characterization of human immunodeficiency virus type 1 from Indonesia.

Journal of acquired immune deficiency syndromes and human retrovirology : official publication of the International Retrovirology Association·1997

The oxygen fixation hypothesis fails to explain oxygen

Area of Science:

  • Radiation biology
  • Molecular biology
  • Biochemistry

Background:

  • The oxygen fixation hypothesis (OFH) explains oxygen's role as a radiation sensitizer.
  • It posits that oxygen-induced DNA lesions are chemically irreparable, increasing cell lethality.
  • This hypothesis has been the leading explanation since the 1950s.

Purpose of the Study:

  • To evaluate the validity of the oxygen fixation hypothesis.
  • To investigate the influence of cellular factors on oxygen-mediated radiation sensitization.

Main Methods:

  • Measured the maximum insignificant dose (MID) in three wild-type strains of Escherichia coli.
  • Assessed the strain-dependent variations in MID values.

Main Results:

Related Experiment Videos

  • Maximum insignificant dose (MID) values exhibited clear strain dependency.
  • This strain dependency contradicts the oxygen fixation hypothesis's prediction of universal chemical restorability.
  • The OFH does not account for the role of enzymatic DNA repair.

Conclusions:

  • The oxygen fixation hypothesis does not fully explain oxygen's role in radiation sensitization.
  • Enzymatic DNA repair mechanisms are crucial and must be considered alongside chemical reactions.
  • Further research should focus on the interplay between radiation chemistry and cellular repair processes.