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Related Experiment Videos

Structure and expression of mitsugumin29 gene

M Shimuta1, S Komazaki, M Nishi

  • 1Department of Pharmacology, Faculty of Medicine, University of Tokyo, Japan.

FEBS Letters
|August 26, 1998
PubMed
Summary
This summary is machine-generated.

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Mitsugumin29, a novel synaptophysin family member, is abundant in skeletal muscle and kidney. This protein may play a role in forming specialized endoplasmic reticulum systems in these tissues.

Area of Science:

  • Molecular Biology
  • Cell Biology
  • Genetics

Background:

  • Mitsugumin29 is a recently identified protein belonging to the synaptophysin family.
  • Synaptophysin family members are characterized by four transmembrane segments and localization to intracellular vesicles.

Purpose of the Study:

  • To isolate and analyze mouse mitsugumin29 cDNA and genomic DNA.
  • To investigate the gene's chromosomal location and evolutionary relationship with the synaptophysin gene.
  • To determine the expression pattern and cellular localization of mitsugumin29.

Main Methods:

  • cDNA and genomic DNA isolation and analysis.
  • Gene mapping using mouse chromosome analysis.
  • RNA blot hybridization and immunoblot analysis for expression studies.

Related Experiment Videos

  • Immunofluorescence microscopy for cellular localization.

    • Main Results:

    • The mitsugumin29 gene is located on mouse chromosome 3 F3-H2 and shares exon-intron organization with the synaptophysin gene.
    • Mitsugumin29 is highly expressed in skeletal muscle and less so in the kidney.
    • In the kidney, mitsugumin29 is specifically found in the cytoplasm of proximal and distal tubule cells.

    Conclusions:

    • Mitsugumin29 exhibits a close evolutionary relationship with the synaptophysin gene.
    • The expression and localization suggest a role for mitsugumin29 in specialized endoplasmic reticulum systems within skeletal muscle and kidney tubule cells.