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Structure and expression of mitsugumin29 gene
M Shimuta1, S Komazaki, M Nishi
1Department of Pharmacology, Faculty of Medicine, University of Tokyo, Japan.
Abstract:
Recently mitsugumin29 unique to the triad junction in skeletal muscle was identified as a novel member of the synaptophysin family; the members of this family have four transmembrane segments and are distributed on intracellular vesicles. In this study, we isolated and analyzed mouse mitsugumin29 cDNA and genomic DNA containing the gene. The mitsugumin29 gene mapped to the mouse chromosome 3 F3-H2 is closely related to the synaptophysin gene in exon-intron organization, which indicates their intimate relationship in molecular evolution. RNA blot hybridization and immunoblot analysis revealed that mitsugumin29 is expressed abundantly in skeletal muscle and at lower levels in the kidney. Immunofluorescence microscopy demonstrated that mitsugumin29 exists specifically in cytoplasmic regions of the proximal and distal tubule cells in the kidney. The results obtained may suggest that mitsugumin29 is involved in the formation of specialized endoplasmic reticulum systems in skeletal muscle and renal tubule cells.
Insights
Mitsugumin29, a novel synaptophysin family member, is abundant in skeletal muscle and kidney. This protein may play a role in forming specialized endoplasmic reticulum systems in these tissues.
Area of Science:
- Molecular Biology
- Cell Biology
- Genetics
Background:
- Mitsugumin29 is a recently identified protein belonging to the synaptophysin family.
- Synaptophysin family members are characterized by four transmembrane segments and localization to intracellular vesicles.
Purpose of the Study:
- To isolate and analyze mouse mitsugumin29 cDNA and genomic DNA.
- To investigate the gene's chromosomal location and evolutionary relationship with the synaptophysin gene.
- To determine the expression pattern and cellular localization of mitsugumin29.
Main Methods:
- cDNA and genomic DNA isolation and analysis.
- Gene mapping using mouse chromosome analysis.
- RNA blot hybridization and immunoblot analysis for expression studies.
- Immunofluorescence microscopy for cellular localization.
Main Results:
- The mitsugumin29 gene is located on mouse chromosome 3 F3-H2 and shares exon-intron organization with the synaptophysin gene.
- Mitsugumin29 is highly expressed in skeletal muscle and less so in the kidney.
- In the kidney, mitsugumin29 is specifically found in the cytoplasm of proximal and distal tubule cells.
Conclusions:
- Mitsugumin29 exhibits a close evolutionary relationship with the synaptophysin gene.
- The expression and localization suggest a role for mitsugumin29 in specialized endoplasmic reticulum systems within skeletal muscle and kidney tubule cells.