Enzyme-linked immunosorbent assay for ondansetron captured from airborne samples

Area of Science:

• Analytical Chemistry
• Occupational Health
• Pharmacology

Background:

• Ondansetron is an anti-emetic drug.
• Monitoring airborne concentrations of ondansetron in the workplace is crucial for occupational health and safety.
• Existing methods for quantifying ondansetron in air samples may lack the necessary speed, sensitivity, or specificity.

Purpose of the Study:

• To develop and optimize a simple, rapid, and reliable enzyme-linked immunosorbent assay (ELISA) for the quantitation of ondansetron.
• To validate the assay for use with air samples collected in occupational settings.
• To assess the suitability of the assay for routine occupational health and hygiene monitoring.

Main Methods:

• Development of an enzyme-linked immunosorbent assay (ELISA) for ondansetron.
• Optimization of the assay for extracted samples from air filters.
• Evaluation of assay performance characteristics including sample throughput, specificity, sensitivity (Limit of Detection - LOD), and precision (Relative Standard Deviation - RSD).

Main Results:

• A simple and rapid ELISA method for ondansetron quantitation was successfully developed.
• The assay demonstrated high sample throughput (40 duplicate samples in 3 hours).
• The method achieved a LOD of 10.5 ng/mL and RSD < 11%, indicating good sensitivity and precision.

Conclusions:

• The developed ELISA method is suitable for determining airborne concentrations of ondansetron.
• The assay meets the requirements for specificity, sensitivity, and precision for occupational health and hygiene monitoring.
• This method provides a valuable tool for assessing workplace exposure to ondansetron.