Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Spatial and temporal growth factor influences on developing midbrain dopaminergic neurons

J Engele1

  • 1Anatomie und Zellbiologie, Universität Ulm, Germany.

Journal of Neuroscience Research
|August 26, 1998
PubMed
Summary

Early embryonic dopaminergic neuron survival is promoted by glial-derived growth factors, while later stages rely on autocrine factors. This study investigates growth factor roles in developing dopaminergic neurons.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Neuroinflammation and Brain Disease.

BMC neurology·2023
Same author

CXCR4 and CXCR7 form a functional receptor unit for SDF-1/CXCL12 in primary rodent microglia.

Neuropathology and applied neurobiology·2013
Same author

Etomidate reduces glutamate uptake in rat cultured glial cells: involvement of PKA.

British journal of pharmacology·2008
Same author

Cyclic AMP differentially regulates the expression of fibroblast growth factor and epidermal growth factor receptors in cultured cortical astroglia.

Neuroscience·2002
Same author

Neurotrophin and GDNF expression increases in rat adrenal glands during experimental colitis.

Neuro endocrinology letters·2002
Same author

Evidence for a ligand-specific signaling through GFRalpha-1, but not GFRalpha-2, in the absence of Ret.

Journal of neuroscience research·2001

Area of Science:

  • Neuroscience
  • Developmental Biology
  • Cell Biology

Background:

  • Dopaminergic neurons are crucial for motor control and cognition.
  • In vitro studies identify numerous growth factors affecting dopaminergic neuron survival and differentiation.
  • The in vivo roles of these growth factors during dopaminergic neuron development remain largely unknown.

Purpose of the Study:

  • To investigate the influence of glial and neuronal factors on dopaminergic cell survival during embryonic development in vivo.
  • To determine the developmental stage-specific roles of growth factors in dopaminergic neuron survival.

Main Methods:

  • Serum-free dissociated cell cultures of embryonic day (E) 15 and E17 rat mesencephalon were used.
  • Cultures were exposed to media conditioned by glia and neurons from mesencephalic, striatal, and cortical regions.

Related Experiment Videos

  • Dopaminergic neuron survival was assessed by counting tyrosine hydroxylase-immunoreactive cells.
  • The effects of specific growth factors (BDNF, GDNF) and a MAP kinase inhibitor (PD98059) were evaluated.

    • Main Results:

    • Glial-conditioned media from mesencephalon, striatum, and cortex significantly increased dopaminergic neuron survival in E15 cultures (up to 2.6-fold).
    • Glial-conditioned media had no effect on dopaminergic neuron survival in E17 cultures.
    • Neuronal-conditioned media from striatal and mesencephalic neurons did not affect E17 dopaminergic neuron survival.
    • E17 dopaminergic neurons remained sensitive to growth factors, as evidenced by PD98059-induced decline in survival and promotion by BDNF and GDNF (1.5- to 1.9-fold).

    Conclusions:

    • Dopaminergic cell survival during early embryonic development is primarily influenced by unknown glial-derived growth factors from the mesencephalon, cortex, and striatum.
    • During later developmental stages, survival is predominantly mediated by autocrine-acting growth factors like BDNF and GDNF.
    • These findings highlight a shift in the regulatory mechanisms of dopaminergic neuron survival throughout embryonic development.