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Related Experiment Videos

Seizure activity induces PIM-1 expression in brain

J D Feldman1, L Vician, M Crispino

  • 1Department of Pediatrics, University of California-Los Angeles, Center for the Health Sciences, USA.

Journal of Neuroscience Research
|August 26, 1998
PubMed
Summary

Forskolin, not depolarization, induces protein kinase PIM-1 (proto-oncogene) expression in PC12 cells. Kainic acid administration increases PIM-1 expression in rat hippocampus, suggesting its role in neuronal stimulation.

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Area of Science:

  • Neuroscience
  • Molecular Biology
  • Oncology

Background:

  • KID-1, a novel protein kinase, is induced by depolarization in PC12 cells and brain.
  • KID-1 shows high sequence homology to PIM-1, a proto-oncogene found in hematopoietic and germ cells.

Purpose of the Study:

  • To investigate PIM-1 expression in stimulated PC12 cells and rat brains.
  • To determine the specific stimuli that induce PIM-1 expression.

Main Methods:

  • Examined PIM-1 expression in PC12 cells treated with forskolin, depolarization, or growth factors.
  • Analyzed PIM-1 mRNA levels in various rat tissues, including brains of kainic acid-treated rats.

Main Results:

  • Forskolin, but not depolarization or growth factors, induced PIM-1 expression in PC12 cells.

Related Experiment Videos

  • PIM-1 was identified as an immediate early gene in response to forskolin.
  • PIM-1 mRNA was detected in unstimulated tissues and at low levels in unstimulated brain.
  • Kainic acid administration significantly induced PIM-1 expression in the dentate gyrus of the hippocampus.

    • Conclusions:

    • PIM-1 expression in PC12 cells is specifically induced by forskolin.
    • PIM-1 is upregulated in the hippocampus following kainic acid-induced stimulation.
    • These findings highlight PIM-1's role as an immediate early gene in neuronal response to specific stimuli.