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Related Experiment Video

Updated: Jul 5, 2026

Microperfusion Technique to Investigate Regulation of Microvessel Permeability in Rat Mesentery

Microperfusion Technique to Investigate Regulation of Microvessel Permeability in Rat Mesentery

Published on: September 12, 2015

PDGF mediates cardiac microvascular communication

J M Edelberg¹, W C Aird, W Wu

¹Department of Medicine, Beth Israel Deaconess Medical Center, Boston, Massachusetts 02215, USA.

The Journal of Clinical Investigation

|August 26, 1998

Summary

This summary is machine-generated.

Cardiac myocytes control endothelial cells via PDGF-AB signaling, regulating von Willebrand factor (vWF) and angiogenesis. This pathway is crucial for cardiac microvascular hemostatic and angiogenic activity.

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Dynamic Measurement and Imaging of Capillaries, Arterioles, and Pericytes in Mouse Heart

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Related Experiment Videos

Last Updated: Jul 5, 2026

Microperfusion Technique to Investigate Regulation of Microvessel Permeability in Rat Mesentery

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Published on: September 12, 2015

Development and Characterization of In Vitro Microvessel Network and Quantitative Measurements of Endothelial [Ca2+]i and Nitric Oxide Production

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Dynamic Measurement and Imaging of Capillaries, Arterioles, and Pericytes in Mouse Heart

Dynamic Measurement and Imaging of Capillaries, Arterioles, and Pericytes in Mouse Heart

Published on: July 29, 2020

Area of Science:

Cardiovascular Biology
Cellular Communication
Angiogenesis Research

Background:

Cardiac myocytes regulate specific endothelial cell functions.
Previous work identified myocyte control over von Willebrand factor (vWF) and a vWF promoter transgene in cardiac microvascular endothelial cells.

Purpose of the Study:

To elucidate the communication pathway between cardiac myocytes and microvascular endothelial cells.
To identify the molecular mechanisms regulating vWF and transgene expression in cardiac endothelial cells.

Main Methods:

Histological examination of cardiac microvasculature.
In vitro transcript and protein analysis of endothelial cells.
Antibody neutralization assays.
In vivo studies using murine embryos.

Main Results:

Cardiac myocytes induce endothelial PDGF-B expression, forming PDGF-AB.
PDGF-AB specifically induces vWF and transgene expression in PDGF alpha-receptor-positive endothelial cells.
This signaling pathway also upregulates vascular endothelial growth factor and Flk-1, promoting angiogenesis.
In vivo antibody neutralization blocked transgene expression.

Conclusions:

A novel communication pathway involving PDGF-AB and its alpha-receptor regulates cardiac microvascular endothelial cell hemostatic and angiogenic activity.
This myocyte-endothelial cell interaction is critical for cardiac microvascular development and function.