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Correlation between contractile strength and myosin heavy chain isoform composition in human skeletal muscle
1Department of Medical Physiology Panum Institute, Denmark p.aagaard@mfi.ku.dk
Purpose:
The purpose of this study was to investigate the relationship between myosin heavy chain (MHC) composition and maximal contraction strength of the human quadriceps femoris muscle.
Methods:
Muscle biopsies were obtained from m. vastus lateralis in your highly physical active males (N = 7). The MHC composition of muscle homogenates was determined by electrophoresis techniques (SDS-PAGE). Isokinetic peak torque and constant-angle torque (50 degrees knee flexion) were obtained during slow (30 degrees.s-1), medium (120 degrees.s-1), and fast (240 degrees.s-1) maximal concentric and eccentric quadriceps contractions and expressed relative to muscle volume.
Results:
The percentage of MHC II in the quadriceps muscle was positively correlated (rs = 0.61-0.93; P < 0.05-0.01) to maximal concentric quadriceps strength obtained at medium to high knee angular velocity. In contrast, no consistent pattern of correlation was observed for maximal eccentric quadriceps strength.
Conclusions:
The relationship observed between muscular MHC composition and maximal contractile strength is suggested to appear as a consequence of MHC -related differences in contractile force-velocity characteristics and/or contractile Rate of Force Development (RFD).