Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Ultrasmall immunogold particles: important probes for immunocytochemistry

J M Robinson1, T Takizawa, D D Vandré

  • 1Department of Cell Biology, Neurobiology, and Anatomy, Ohio State University, Columbus 43210, USA.

Microscopy Research and Technique
|August 26, 1998
PubMed
Summary

This review compares conventional colloidal gold and ultrasmall gold particles for immunocytochemistry. Ultrasmall gold particles offer advantages in specific applications, enhancing immunolabeling techniques.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Flavor-specific enhancement of electronic cigarette liquid consumption and preference in mice.

Drug and alcohol dependence·2020
Same author

Differential gene expression and gene-set enrichment analysis in Caco-2 monolayers during a 30-day timeline with Dexamethasone exposure.

Tissue barriers·2019
Same author

Modelling the structure of a ceRNA-theoretical, bipartite microRNA-mRNA interaction network regulating intestinal epithelial cellular pathways using R programming.

BMC research notes·2018
Same author

Ultrastable Silicon Cavity in a Continuously Operating Closed-Cycle Cryostat at 4 K.

Physical review letters·2017
Same author

A Fermi-degenerate three-dimensional optical lattice clock.

Science (New York, N.Y.)·2017
Same author

1.5  μm Lasers with Sub-10 mHz Linewidth.

Physical review letters·2017

Area of Science:

  • Biotechnology
  • Immunocytochemistry
  • Microscopy

Background:

  • Immunocytochemistry utilizes immunoprobes for detecting antigens in cells and tissues.
  • Conventional colloidal gold particles and ultrasmall gold particles are common immunoprobes.
  • Understanding their comparative performance is crucial for optimizing experimental outcomes.

Purpose of the Study:

  • To review and compare conventional colloidal gold and ultrasmall gold particles as immunoprobes in immunocytochemistry.
  • To discuss the advantages and disadvantages of each particle type.
  • To present experimental findings comparing these immunoprobes and explore fluorescently labeled ultrasmall probes for correlative microscopy.

Main Methods:

  • Literature review of immunocytochemical studies.

Related Experiment Videos

  • Comparative analysis of conventional and ultrasmall gold particle immunoprobes.
  • Experimental validation in selected laboratory settings.

    • Main Results:

    • Ultrasmall gold particles demonstrate distinct advantages in certain immunocytochemical applications.
    • Comparative studies highlight specific scenarios where one probe type outperforms the other.
    • Development and evaluation of fluorescently labeled ultrasmall immunoprobes for advanced imaging.

    Conclusions:

    • The choice between conventional and ultrasmall gold particles depends on specific experimental needs.
    • Ultrasmall gold particles offer enhanced sensitivity and penetration in some immunocytochemical protocols.
    • Fluorescently labeled ultrasmall probes provide novel opportunities for correlative microscopy.