Related Experiment Videos
Developmental pattern of plasminogen activator activity in chick brain hemispheres
G Scicolone1, S Pereyra-Alfonso, J L Ferrán
1Institute of Cell Biology and Neurosciences Prof. Eduardo De Robertis, School of Medicine, University of Buenos Aires, Argentine. gscicolo@mail.retina.ar
Neurochemical Research
|August 26, 1998
Summary
Developing chick brain hemispheres show a unique temporal pattern of urokinase-type plasminogen activator activity, distinct from general developmental markers. This suggests specific regulatory functions during brain development.
Area of Science:
- Developmental Biology
- Neuroscience
- Biochemistry
Background:
- Plasminogen activators are crucial for various developmental processes.
- Previous studies identified developmental protease activity patterns in chick optic lobe and cerebellum.
Purpose of the Study:
- To investigate the temporal development pattern of plasminogen activator activity in chick brain hemispheres.
- To identify the specific type of plasminogen activator present during brain development.
Main Methods:
- Assayed plasminogen activator activity in soluble fractions from membrane preparations using a radial fibrinolytic assay.
- Utilized enzyme inhibitors and antibodies against plasminogen activators for enzyme identification.
- Compared activity patterns with general developmental parameters.
Main Results:
- Developing brain hemispheres exclusively express urokinase-type plasminogen activator.
- The temporal pattern of this activity differs significantly from general developmental parameters.
- The urokinase-type plasminogen activator activity exhibits a distinct developmental regulation.
Conclusions:
- Plasminogen activator activity in developing brain hemispheres is primarily urokinase-type.
- This activity is developmentally regulated and follows a unique temporal pattern.
- The findings suggest specific roles for plasminogen activators in distinct stages of brain development.