Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Immunological and functional differences between cord and peripheral blood

S B Cohen1, J A Madrigal

  • 1The Anthony Nolan Research Institute and the Department of Haematology, The Royal Free Hospital, Hampstead, London, UK.

Bone Marrow Transplantation
|August 26, 1998
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Evaluation of a machine learning tool for the early identification of patients with undiagnosed psoriatic arthritis - A retrospective population-based study.

Journal of translational autoimmunity·2023
Same author

Assessing vaccine-mediated protection in an ultra-low dose <i>Mycobacterium tuberculosis</i> murine model.

bioRxiv : the preprint server for biology·2023
Same author

Correction to: PANLAR consensus statement on biosimilars.

Clinical rheumatology·2019
Same author

PANLAR consensus statement on biosimilars.

Clinical rheumatology·2019
Same author

High-resolution HLA allele and haplotype frequencies in majority and minority populations of Costa Rica and Nicaragua: Differential admixture proportions in neighboring countries.

HLA·2018
Same author

Single molecule real-time DNA sequencing of HLA genes at ultra-high resolution from 126 International HLA and Immunogenetics Workshop cell lines.

HLA·2017
Same journal

CHIME: a novel HLA eplet model associates with non-relapse mortality in haploidentical hematopoietic cell transplant with post-transplant cyclophosphamide.

Bone marrow transplantation·2026
Same journal

Transplant outcomes for patients with severe acquired aplastic anemia over the age of 40 using busulfan, fludarabine, reduced-dose cyclophosphamide, and anti-thymocyte globulin conditioning regimen.

Bone marrow transplantation·2026
Same journal

A holistic prognostic model for leukemia-free survival after allogeneic transplantation in acute leukemia.

Bone marrow transplantation·2026
Same journal

Early software-assisted response predicts survival in a prospective cohort of 258 newly diagnosed cGvHD patients.

Bone marrow transplantation·2026
Same journal

Evaluation of a novel eHealth-facilitated, post HCT-integrated care model on implementation and rehospitalization: results from the Swiss SMILe hybrid-RCT.

Bone marrow transplantation·2026
Same journal

Impact of doublet post-transplant maintenance on outcomes in multiple myeloma: A propensity score matching analysis.

Bone marrow transplantation·2026
See all related articles

Cord blood (CB) transplantation offers advantages over bone marrow (BM) transplants, potentially due to unique serum factors influencing T cell proliferation and reducing graft-versus-host disease (GVHD).

Area of Science:

  • Immunology
  • Transplantation Biology

Background:

  • Cord blood (CB) transplantation is a promising alternative to bone marrow (BM) transplantation, with ongoing debate regarding reduced graft-versus-host disease (GVHD) and increased HLA mismatch tolerance.
  • The immunological differences between CB and adult cells, particularly monocytes, are being investigated to explain transplant outcomes.
  • Reduced CB cell function is often attributed to lymphocyte naiveté, characterized by higher CD4+ CD45RA+ cells and lower T cell proliferation and cytokine production.

Purpose of the Study:

  • To investigate the role of serum factors in the functional differences between cord blood and adult lymphocytes.
  • To determine if serum composition influences T cell proliferation responses in vitro.
  • To explore how these serum-mediated effects might explain the lower incidence of GVHD in CB transplantation.

Related Experiment Videos

Main Methods:

  • Analysis of T cell proliferation responses to mitogens and cytokines using heat-inactivated sera from cord blood and adult donors.
  • Utilizing adult peripheral blood mononuclear cells for in vitro functional assays.
  • Comparison of proliferative responses in the presence of adult serum versus CB serum.

Main Results:

  • Adult serum significantly enhanced T cell proliferative responses.
  • Cord blood serum had no significant effect on T cell proliferation.
  • These findings suggest the presence of a T cell proliferation-enhancing factor in adult serum that is absent in cord blood serum.

Conclusions:

  • A factor present in mature adult serum, but absent in fetal serum, plays a role in T cell proliferation.
  • The absence of this factor in CB serum may explain the reduced proliferative capacity of CB T cells.
  • This serum factor difference could contribute to the lower incidence of GVHD observed in cord blood transplantation.