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Ferrous iron uptake in Cryptococcus neoformans

E S Jacobson1, A P Goodner, K J Nyhus

  • 1Research Service, McGuire Veterans Affairs Medical Center, Richmond, Virginia 23249, and Department of Internal Medicine, Virginia Commonwealth University, Richmond, Virginia 23298-0049, USA. jacobson.eric_s@richmond.va.gov

Infection and Immunity
|August 26, 1998
PubMed
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Cryptococcus neoformans utilizes a reductive iron uptake pathway, with distinct high- and low-affinity systems. Iron uptake requires metabolic energy and primarily transports the Fe(II) species.

Area of Science:

  • Microbiology
  • Molecular Biology
  • Biochemistry

Background:

  • Ferric reduction is implicated in the iron uptake pathway of Cryptococcus neoformans.
  • Understanding iron acquisition is crucial for studying this opportunistic pathogen.

Purpose of the Study:

  • To directly investigate the iron uptake mechanisms in Cryptococcus neoformans.
  • To characterize the kinetics and regulation of iron transport systems.

Main Methods:

  • Direct iron uptake assays using radioactive 55Fe in the presence of various reductants.
  • Kinetic analysis of iron uptake at different concentrations and conditions.
  • Investigating the role of metabolic energy, ferric reductase, and iron species (Fe(II)) in uptake.

Main Results:

Related Experiment Videos

  • Identified both high- and low-affinity iron uptake systems, with a Km of 0.6 microM Fe(II) for high-affinity uptake.
  • High-affinity iron uptake is energy-dependent, decreased by iron repletion and copper starvation, and primarily transports Fe(II).
  • Cell membrane ferric reductase activity contributes significantly to iron uptake.

Conclusions:

  • Cryptococcus neoformans employs a reductive iron uptake pathway, predominantly transporting Fe(II).
  • The pathogen possesses distinct high- and low-affinity iron transporters regulated by cellular conditions.
  • Iron uptake is an active, energy-dependent process involving cell membrane ferric reductase.