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Evaluation and additional recommendations for preparing a whole blood control material

N E Fink1, A Fernández Alberti, I Crispini

  • 1Departamento de Ciencias Biológicas, Facultad de Ciencias Exactas, Universidad Nacional de La Plata, Argentina. nfink@netverk.com.ar

Revista De Saude Publica
|August 26, 1998
PubMed
Summary
This summary is machine-generated.

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A new, low-cost hematology control material was developed. It demonstrates acceptable reproducibility for both manual and automated methods when stored properly, ensuring reliable quality control.

Area of Science:

  • Clinical Hematology
  • Laboratory Medicine
  • Quality Control

Background:

  • Accurate hematology testing relies on stable control materials.
  • Existing control materials can be costly or difficult to prepare.
  • Need for an accessible and reliable control for diverse laboratory settings.

Purpose of the Study:

  • To develop and assess an easy-to-prepare, low-cost control material for hematology.
  • To evaluate the stability and aliquot variability of this material using both manual and automated methods.

Main Methods:

  • Stabilized whole blood aliquots were prepared using partial aldehyde fixation.
  • Stability was tested at 4, 20, and 37 degrees C for up to 9 weeks.
  • Aliquot variability was assessed using both manual and automated hematology analyzers.

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Main Results:

  • Automated methods showed low coefficient of variation (CV) for most parameters (e.g., RBC 0.7%, Hb 0.6%).
  • Manual methods exhibited higher CVs (e.g., WBC 23%, PLT 41%).
  • Material remained stable at 4 and 20 degrees C but degraded rapidly at 37 degrees C.

Conclusions:

  • The developed control material is stable and suitable for quality control when protected from high temperatures during distribution.
  • It offers acceptable reproducibility for both manual and automated hematology methods.
  • This provides a cost-effective option for internal and external quality assessment.