Differentiation of T lymphocytes in the human adenoid as measured by the expression of CD45 isoforms

Encounter of antigen by T lymphocyte on antigen-presenting cells results in changes in the expression of several cell surface molecules, including the abundant cell surface glycoprotein CD45. We have characterized the expression of the CD45 isoforms CD45RA and CD45RO in CD4+ and CD8+ T lymphocytes in the adenoids and peripheral blood of young children. We found that the relative proportions of CD45RA-,CD45RO+ antigen-experienced T cells was higher in the adenoids than in peripheral blood, and that the proportion of naive or resting CD45RA+,CD45RO- T cells was lower in the adenoids than in peripheral blood. The frequency of bright double-positive CD45RA+,CD45RO+ T cells, which represent cells in transition from the CD45RA+ to CD45RO+ phenotype, was higher in the adenoids than in peripheral blood. The frequency of another double-positive cell population, but with unknown ontogeny, expressing both CD45RA and CD45RO at a low level, was higher in peripheral blood than in adenoidal T cells. It was found that the frequency of adenoidal antigen-experienced CD45RA-,CD45RO+ T lymphocytes increased with increasing age of the child. These results are consistent with the model that the adenoids serve as a site for conversion of CD45RA+ to CD45RO+ T lymphocytes, and that the maturation of the immune system in young children is associated with phenotypic changes in T lymphocytes residing in secondary lymphoid organs.