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Inferences about the catalytic domain of P-type ATPases from the tertiary structures of enzymes that catalyze the
I N Smirnova1, V N Kasho, L D Faller
1Department of Medicine, University of California at Los Angeles School of Medicine, Department of Veterans Affairs Medical Center, West Los Angeles 90073, USA.
Abstract:
The machinery to catalyze elementary reactions is conserved, and the number of solved enzyme structures is increasing exponentially. Therefore, structures of enzymes that catalyze phosphate transfer are reviewed, and a supersecondary structure connecting the Walker A sequence to another sequence containing functional amino acids is proposed as an additional signature for the active site. The new signature is used to infer the identity of the P-loop in P-type biological pumps and may be useful in predicting targets for site-directed mutagenesis in other enzymes of unknown structure like the AAA family and ABC transporters.