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Biochemical Reconstitution of Steroid Receptor•Hsp90 Protein Complexes and Reactivation of Ligand Binding
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Coactivation and corepression in transcriptional regulation by steroid/nuclear hormone receptors

J D Chen1, H Li

  • 1Department of Pharmacology and Molecular Toxicology, University of Massachusetts Medical School, Worcester 01655-0126, USA.

Critical Reviews in Eukaryotic Gene Expression
|August 26, 1998
PubMed

Insights

Hormone action involves steroid/nuclear receptors regulating gene expression. These receptors interact with corepressors or coactivators to control chromatin structure and gene activity, impacting cell differentiation and homeostasis.

Area of Science:

  • Molecular Biology
  • Endocrinology
  • Epigenetics

Background:

  • Steroid/nuclear receptors are crucial for hormone action, controlling cell differentiation, development, and homeostasis.
  • Understanding the molecular mechanisms of transcriptional regulation by these receptors is essential.

Purpose of the Study:

  • To investigate the mechanisms of gene activation and repression by steroid/nuclear receptors.
  • To identify and characterize new proteins involved in this signaling pathway.

Main Methods:

  • Identification and cloning of novel proteins modulating transcription with nuclear receptors.
  • Analysis of corepressor and coactivator complex interactions with nuclear receptors.

Main Results:

  • Unliganded receptors interact with corepressors (SMRT, N-CoR) targeting histone deacetylases, leading to repressed chromatin.
  • Hormone binding triggers replacement of corepressors with coactivators (SRC1, TIF2, RAC3, CBP, p300) targeting histone acetyltransferases, creating accessible chromatin.

Conclusions:

  • These findings reveal novel entry points for understanding transcriptional regulation by steroid/nuclear receptors.
  • The dynamic interplay between receptors, corepressors, and coactivators is central to hormone-mediated gene control.

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