Antibacterial activity in Pleurochaete squarrosa extract (Bryophyta)

Area of Science:

• * Botany
• * Microbiology
• * Pharmacology

Background:

• * Mosses are a rich source of bioactive compounds.
• * Pleurochaete squarrosa is a bryophyte with potential medicinal properties.
• * Gram-negative bacteria pose significant public health challenges due to antibiotic resistance.

Purpose of the Study:

• * To investigate the antibacterial potential of Pleurochaete squarrosa acetone extract.
• * To determine the efficacy of the extract against pathogenic bacterial strains.
• * To compare the extract's activity with existing antibiotics and assess its toxicity.

Main Methods:

• * An acetone extract of Pleurochaete squarrosa was prepared.
• * Broth microdilution method was used to determine Minimum Inhibitory Concentrations (MICs).
• * Antibacterial activity was tested against eleven bacterial strains, including Gram-negative pathogens.
• * Acute toxicity was evaluated in Balb C mice.

Main Results:

• * The Pleurochaete squarrosa extract demonstrated activity against some Gram-negative bacterial strains.
• * Minimum Inhibitory Concentrations (MICs) were determined for the active extract.
• * Comparative analysis showed varying efficacy against reference antibiotics.
• * Acute toxicity assays in mice provided preliminary safety data.

Conclusions:

• * Pleurochaete squarrosa extract possesses notable antibacterial properties, particularly against Gram-negative bacteria.
• * The extract shows promise as a potential source for novel antibacterial agents.
• * Further investigation into the active compounds and therapeutic applications is warranted.