Related Experiment Video

Updated: Sep 6, 2026

Induction of an Inflammatory Response in Primary Hepatocyte Cultures from Mice
08:32

Induction of an Inflammatory Response in Primary Hepatocyte Cultures from Mice

Published on: March 10, 2017

Induction of IL-15 mRNA and protein in A549 cells by pro-inflammatory cytokines

M Stoeck, W Kromer, V Gekeler

    Immunobiology
    |August 26, 1998
    PubMed

    Insights

    Interleukin-15 (IL-15) mRNA and protein are constitutively expressed in human lung carcinoma cells. Tumor necrosis factor-alpha and IL-1 beta increase IL-15 expression, suggesting targets for cancer therapy.

    Area of Science:

    • Immunology
    • Molecular Biology
    • Cancer Research

    Background:

    • Interleukin-15 (IL-15) is a cytokine with functional similarities to IL-2.
    • Understanding IL-15 expression is crucial for identifying therapeutic targets.

    Purpose of the Study:

    • To investigate the expression of IL-15 mRNA and protein in the A549 human lung carcinoma cell line.
    • To identify factors that modulate IL-15 expression in this cell line.

    Main Methods:

    • Quantitative analysis of IL-15 mRNA expression using RT-PCR.
    • Detection of IL-15 protein in cell lysates and supernatants.
    • Treatment of A549 cells with various cytokines including TNF-alpha, IL-1 beta, and IFN-gamma, as well as growth factors like EGF and KGF.

    Main Results:

    • Constitutive expression of IL-15 mRNA was observed in A549 cells.
    • Treatment with TNF-alpha or IL-1 beta (10 ng/ml) resulted in an approximate 2-fold increase in IL-15 mRNA.
    • IFN-gamma significantly affected IL-15 mRNA expression only at higher concentrations (100 ng/ml).
    • Combinations of TNF-alpha and IFN-gamma did not yield superior results compared to TNF-alpha alone.
    • EGF and KGF, individually or in combination, did not affect IL-15 expression.
    • IL-15 protein was detected in cell lysates of unstimulated cells and its levels increased upon stimulation with TNF-alpha or IL-1 beta.
    • No significant IL-15 protein was found in the cellular supernatants.

    Conclusions:

    • A549 lung carcinoma cells express IL-15 constitutively.
    • IL-15 expression is upregulated by pro-inflammatory cytokines TNF-alpha and IL-1 beta.
    • These findings highlight potential pathways for modulating IL-15 in lung cancer.

    More Related Videos

    Advanced Imaging of Lung Homing Human Lymphocytes in an Experimental In Vivo Model of Allergic Inflammation Based on Light-sheet Microscopy10:39

    Advanced Imaging of Lung Homing Human Lymphocytes in an Experimental In Vivo Model of Allergic Inflammation Based on Light-sheet Microscopy

    Published on: April 16, 2019

    Primary Sjogren's Syndrome Associated with Lung Adenocarcinoma: Probing the Potential Common Pathogenic Mechanisms and Experimental Verification10:21

    Primary Sjogren's Syndrome Associated with Lung Adenocarcinoma: Probing the Potential Common Pathogenic Mechanisms and Experimental Verification

    Published on: September 20, 2024

    Related Concept Videos

    T Cell Types and Functions01:24

    T Cell Types and Functions

    When T cells with CD4 markers are activated, they give rise to two types of effector cells: helper T cells and regulatory T cells. Meanwhile, T cells with CD8 markers differentiate into effector cytotoxic T cells. The differentiation of CD4 T cells into helper T cell subsets, such as Th1, Th2, and Th17 cells, is dependent on the antigen type, antigen-presenting cell, and regulatory cytokines.
    Th1 cells stimulate dendritic cells to express necessary co-stimulatory molecules on their surfaces for...
    Chronic Inflammation: Introduction01:12

    Chronic Inflammation: Introduction

    Chronic inflammation is a prolonged, dysregulated immune response that persists for weeks to years when the inciting stimulus is difficult to eradicate or when self‑antigens drive ongoing reactivity. Morphologically, it is defined by mononuclear cell infiltration, progressive tissue destruction, and concurrent attempts at healing via angiogenesis and fibrosis. Compared with acute inflammation, edema is less prominent while cellular infiltration predominates; triggers include persistent...