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Related Experiment Videos

Changes in conformation and stability upon SCF/sKit complex formation

L O Narhi1, J Wypych, T Li

  • 1Amgen Inc., Amgen Center, Thousand Oaks, California 91320-1789, USA. lnarhi@amgen.com

Journal of Protein Chemistry
|August 26, 1998
PubMed
Summary
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Stem cell factor (SCF) binding to its receptor Kit induces conformational changes in both molecules. These structural alterations in SCF and soluble Kit (sKit) are crucial for receptor dimerization and subsequent signal transduction.

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Structural Biology

Background:

  • Stem cell factor (SCF) is a four-helical bundle cytokine and the ligand for the Kit tyrosine kinase receptor.
  • SCF/Kit interaction is vital for hematopoietic progenitors, melanocytes, and mast cells.
  • Kit receptor dimerization and transphosphorylation occur upon SCF binding.

Purpose of the Study:

  • To investigate the conformational changes in SCF and soluble Kit (sKit) upon their binding.
  • To elucidate the role of these conformational changes in signal transduction.

Main Methods:

  • Circular dichroism (CD) spectroscopy (near- and far-UV).
  • Fourier transform infrared (FTIR) spectroscopy.
  • Intrinsic fluorescence spectroscopy.

Related Experiment Videos

  • Analysis of thermal denaturation.

    • Main Results:

    • Ligand/receptor binding induced reproducible conformational changes in SCF and sKit.
    • Far-UV CD and FTIR indicated a slight increase in alpha-helical content.
    • Near-UV CD and fluorescence spectra revealed alterations in aromatic amino acid environments.
    • SCF thermal denaturation was unaffected; sKit melting temperature slightly increased upon binding.
    • Binding is temperature-dependent with a significant enthalpy of binding.

    Conclusions:

    • SCF/sKit binding results in subtle but significant conformational changes.
    • These conformational shifts likely mediate Kit receptor dimerization and downstream signal transduction.
    • Structural insights into SCF/Kit interactions are essential for understanding cellular signaling pathways.