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Related Experiment Videos

Lactase activity of microorganisms

M V Rao, S M Dutta

    Folia Microbiologica
    |January 1, 1978
    PubMed
    Summary
    This summary is machine-generated.

    Researchers screened 62 microbial strains for lactase (beta-D-galactosidase) activity. Bacteria showed the highest enzyme activity and yield, with specific strains of Streptococcus, Lactobacillus, and Leuconostoc identified as promising sources.

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    Area of Science:

    • Microbiology
    • Enzymology
    • Biotechnology

    Background:

    • Lactase (beta-D-galactosidase) is crucial for lactose digestion.
    • Identifying microbial sources of lactase is important for industrial applications.
    • Screening diverse microorganisms can reveal novel enzyme activities.

    Purpose of the Study:

    • To screen various yeast, mold, and bacteria strains for lactase activity.
    • To evaluate the cell yield and specific enzyme activity of promising strains.
    • To identify high-performing microbial candidates for lactase production.

    Main Methods:

    • Screening of 62 microbial strains (yeasts, molds, bacteria) for lactase activity.
    • Quantification of cell yield and enzyme units (per liter, per g dry weight, per mg protein).

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  • Evaluation of cell-free extracts for enzyme characterization.

    • Main Results:

    • Bacteria exhibited the highest lactase activity and lowest cell yield.
    • Molds showed the lowest enzyme activity but the highest cell yields.
    • Saccharomyces fragilis, Streptococcus cremoris, Lactobacillus bulgaricus, and Leuconostoc citrovorum demonstrated very high lactase activity.

    Conclusions:

    • Bacteria are superior sources for high lactase enzyme activity compared to yeasts and molds.
    • Specific strains like Lactobacillus bulgaricus and Streptococcus cremoris are excellent candidates for industrial lactase production.
    • Further research can optimize conditions for these high-activity strains.