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Electrophoretic analyses in a case of monoclonal gamma chain disease
J D Tissot1, A Tridon, M Ruivard
1Service régional vaudois de transfusion sanguine, Lausanne, Switzerland. jean-daniel.tissot@chuv.hospvd.ch
Electrophoresis
|August 27, 1998
Abstract:
Abnormal low molecular weight immunoglobulin heavy chain can be detected in serum samples from patients with heavy chain disease. In this paper, we report the characterization of a gamma heavy chain in a patient suffering from a lymphoplasmocytic disorder, using immunofixation analysis and two-dimensional polyacrylamide gel electrophoresis. We demonstrate that the combination of serum protein agarose electrophoresis and two-dimensional electrophoresis (three-dimensional electrophoresis) can be used to further characterize abnormal protein bands detected by immunofixation.