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Vibrio cholerae adherence and colonization in experimental cholera: electron microscopic studies
Infection and Immunity
|August 1, 1976
Summary
Vibrio cholerae colonization of rabbit intestines shows a lag before attachment, peaking at 4-7 hours, then rapidly clearing. Infant rabbits exhibit uniform, immediate attachment without a lag period.
Area of Science:
- Microbiology
- Gastroenterology
- Pathogen-Host Interactions
Background:
- Vibrio cholerae is a significant cause of bacterial gastroenteritis.
- Understanding the dynamics of V. cholerae colonization is crucial for developing effective treatments.
Purpose of the Study:
- To investigate the colonization patterns of Vibrio cholerae in rabbit intestinal models.
- To elucidate the temporal dynamics of bacterial attachment and clearance.
Main Methods:
- Utilized ligated ileal loops in adult rabbits and the patent gut of infant rabbits.
- Employed scanning and transmission electron microscopy for detailed visualization.
- Conducted time-course studies to observe colonization progression.
Main Results:
- Adult rabbit model showed a 1-hour lag before V. cholerae attachment, with peak colonization at 4-7 hours and rapid clearance thereafter.
- Infant rabbit model demonstrated uniform, immediate attachment without a lag period.
- Bacteria adhered to microvilli tips, with some embedding into the brush border, causing microvillar changes.
Conclusions:
- V. cholerae colonization follows distinct temporal patterns in different rabbit intestinal models.
- Attachment mechanisms involve surface coat interaction with microvilli.
- Potential role of globular protrusions in bacterial clearance observed.