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[Cloning and biochemical identification of the ribR gene in Bacillus subtilis]
I M Solov'eva1, R A Kreneva, B M Polanuer
1Department of Molecular and Radiation Biophysics, Konstantinov Institute of Nuclear Physics, Russian Academy of Sciences, Gatchina, Leningrad oblast', Russia.
Genetika
|August 28, 1998
Abstract:
The PCR copy of the ribR gene of Bacillus subtilis was subcloned in Escherichia coli cells under the control of the phage T7 inducible promoter. The polypeptide of 26 kDa corresponding to the 690-bp gene is the product of the ribR gene. The protein encoded by the ribR gene is flavokinase, and the riboflavin-reduced form is the substrate for it.