Direct evidence for an angiotensin AT1 receptor type in rat vas deferens

Physiological experiments suggest that the angiotensin AT1 receptor type predominates in rat vas deferens. Membrane binding experiments, using 125I-[Sarl,Ile8]angiotensin II, confirm the presence of angiotensin AT1 receptors and the absence of angiotensin AT2 receptors in this tissue. Angiotensin II and the angiotensin AT1 receptor-specific antagonist, losartan, bind to rat vas deferens membranes with comparable affinity, with KD equal to 22.7 and 34.1 nM, respectively. The affinities of angiotensin AT2 receptor-specific ligands are 3 orders of magnitude lower. According to the numbers of binding sites and Western blotting of membrane proteins, the concentration of angiotensin AT1 receptors in the rat vas deferens is rather low. The fact that similar numbers of binding sites were obtained from binding data for angiotensin II and losartan further supports the hypothesis of exclusive existence of angiotensin AT1 receptor type in rat vas deferens.