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Generation of Discriminative Human Monoclonal Antibodies from Rare Antigen-specific B Cells Circulating in Blood
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Humanized monoclonals and other biological initiatives

P F Halloran1, S Prommool

  • 1Department of Medicine, University of Alberta, Edmonton, Canada. phil.halloran@ualberta.ca

Clinical Biochemistry
|August 29, 1998
PubMed
Abstract

No abstract available in PubMed .

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Hybridoma Technology01:31

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Hybridoma technology is used for the large-scale production of monoclonal antibodies. Monoclonal antibodies bind to only a single antigenic determinant or epitope. Such antibodies are used in research, diagnostics, and disease therapy. The hybridoma technology established in 1975 by Georges Köhler and Cesar Milstein was awarded the Nobel Prize in Medicine in 1984 for revolutionizing research and therapy.
Hybridoma Selection
Commonly used fusion techniques — electroporation, polyethylene glycol...