Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Basement membrane proteins influence brain capillary endothelial barrier function in vitro

T Tilling1, D Korte, D Hoheisel

  • 1Institut für Biochemie, Westfälische Wilhelms-Universität Münster, Germany.

Journal of Neurochemistry
|August 29, 1998
PubMed
Summary

Basement membrane proteins like type IV collagen, fibronectin, and laminin significantly enhance the barrier function of brain endothelial cells. These proteins improve the reliability of in vitro models for the blood-brain barrier.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Intestinal formation of trans-crocetin from saffron extract (Crocus sativus L.) and in vitro permeation through intestinal and blood brain barrier.

Phytomedicine : international journal of phytotherapy and phytopharmacology·2015
Same author

Phenotypic changes in the fluidity of the tonoplast membrane of crassulacean-acid-metabolism plants in response to temperature and salinity stress.

Planta·2013
Same author

A new astrocytic cell line which is able to induce a blood-brain barrier property in cultured brain capillary endothelial cells.

Cytotechnology·2012
Same author

Quantification of the Interaction between Charged Guest Molecules and Chemisorbed Monothiolated β-Cyclodextrins.

Analytical chemistry·2011
Same author

Chronic stress induces opposite changes in the mRNA expression of the cell adhesion molecules NCAM and L1.

Neuroscience·2002
Same author

Predicting blood-brain barrier permeability of drugs: evaluation of different in vitro assays.

Journal of drug targeting·2002

Area of Science:

  • Cell Biology
  • Biophysics
  • Neuroscience

Background:

  • Primary cultures of porcine brain capillary endothelial cells are used to model the blood-brain barrier.
  • Basement membrane proteins play a crucial role in cellular structure and function.

Purpose of the Study:

  • To investigate the influence of specific basement membrane proteins on the cellular barrier properties of brain endothelial cells.
  • To assess the suitability of these proteins for enhancing in vitro blood-brain barrier models.

Main Methods:

  • Cells were cultured on various substrates including type IV collagen, fibronectin, laminin, and mixtures thereof.
  • Transcellular electrical resistance (TER) was measured using impedance spectroscopy.
  • Rat tail collagen served as a reference substratum.

Related Experiment Videos

Main Results:

  • Type IV collagen, fibronectin, and laminin significantly increased TER (2.3- to 2.9-fold) in cells with low baseline resistance.
  • The increase in TER was less pronounced (1.1- to 1.2-fold) in cells with high baseline resistance.
  • A mixture of type IV collagen and SPARC did not elevate TER.

Conclusions:

  • Type IV collagen, fibronectin, and laminin are implicated in the formation of tight junctions in cerebral capillary endothelial cells.
  • These proteins enhance the reliability and suitability of primary microvascular endothelial cell cultures as an in vitro blood-brain barrier model.
  • The observed effects suggest that basement membrane proteins can modulate endothelial cell differentiation.