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Related Experiment Videos

Calbindin D28k expression in immunohistochemically identified Renshaw cells

P A Carr1, F J Alvarez, E A Leman

  • 1Department of Anatomy, Wright State University, Dayton, OH 45435, USA.

Neuroreport
|August 29, 1998
PubMed
Summary

Renshaw cells in rats universally express calbindin D28k, a calcium-binding protein. In cats, this expression is less common, suggesting species-specific homeostatic mechanisms in these spinal cord neurons.

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Area of Science:

  • Neuroscience
  • Cell Biology
  • Spinal Cord Research

Background:

  • Renshaw cells are inhibitory interneurons in the spinal cord.
  • Their role in motor control is well-established.
  • The molecular mechanisms underlying their function, particularly calcium handling, are not fully understood.

Purpose of the Study:

  • To investigate the presence of calbindin D28k immunoreactivity in Renshaw cells.
  • To compare the expression patterns of calbindin D28k in Renshaw cells between rats and cats.
  • To explore potential links between calbindin D28k expression and Renshaw cell function.

Main Methods:

  • Double immunofluorescence staining was used to detect calbindin D28k and gephyrin.
  • Renshaw cells were identified by gephyrin immunoreactivity and characteristic morphology.

Related Experiment Videos

  • Immunoreactivity was analyzed in lumbar spinal cord sections of rats and cats.

    • Main Results:

    • In rats, all identified Renshaw cells (n=487) showed calbindin D28k immunoreactivity.
    • In cats, 47% (n=128) of Renshaw cells exhibited calbindin D28k immunoreactivity.
    • Occasional calbindin D28k-immunoreactive Renshaw cells were found in lamina IX in both species.

    Conclusions:

    • Calbindin D28k is a common marker for Renshaw cells in rats, suggesting a role in calcium buffering.
    • Species-specific differences in calbindin D28k expression indicate varied homeostatic mechanisms in Renshaw cells.
    • High-rate firing of Renshaw cells likely necessitates efficient intracellular calcium regulation.