Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Gene mapping in zebrafish using single-strand conformation polymorphism analysis

D Förnzler1, H Her, E W Knapik

  • 1Genetics Division, Brigham and Women's Hospital, Harvard Medical School, Boston, Massachusetts 02115, USA.

Genomics
|September 2, 1998
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Artificial intelligence in CT for predicting lymph node metastasis in rectal cancer patients: a meta-analysis.

Clinical radiology·2025
Same author

Hepatic gene expression variations in response to high-fat diet-induced impaired glucose tolerance using RNAseq analysis in collaborative cross mouse population.

Mammalian genome : official journal of the International Mammalian Genome Society·2019
Same author

Effect of Linearization in a WNT Signaling Model.

Computational and mathematical methods in medicine·2019
Same author

The bromodomain protein BRD4 regulates the KEAP1/NRF2-dependent oxidative stress response.

Cell death & disease·2014
Same author

A mutation in Tubb2b, a human polymicrogyria gene, leads to lethality and abnormal cortical development in the mouse.

Human molecular genetics·2013
Same author

Molecular characterization of signalling pathways in cancer stem cells.

Ecancermedicalscience·2012
Same journal

Integrating transcriptomics and metabolomics reveals the molecular landscape of sperm maturation driven by regional differentiation in the epididymis of Guizhou-Guiqian semi-fine wool sheep.

Genomics·2026
Same journal

Impact of genotype on histopathology and clinical characters in a Chinese cohort with obstructive hypertrophic cardiomyopathy.

Genomics·2026
Same journal

A novel reusable transcriptome-wide association study workflow used to map key genes linked to important cattle traits.

Genomics·2026
Same journal

The large mitochondrial genome of Syndiclis anlungensis (Lauraceae): Genome structure, comparative analysis, and phylogenetic relationships with other Syndiclis species.

Genomics·2026
Same journal

DeepGEP: Deep learning for gene expression prediction from multi-omics in mammals.

Genomics·2026
Same journal

Molecular features of external Auditory Canal cholesteatoma by microbial metagenomic sequencing.

Genomics·2026
See all related articles

Single-strand conformation polymorphism analysis (SSCP) is an efficient tool for gene mapping in zebrafish. This method aids in high-resolution mapping of transcribed sequences, enhancing genomic analysis for vertebrate development studies.

Area of Science:

  • Genomics
  • Developmental Biology
  • Comparative Genomics

Background:

  • The zebrafish is a powerful model organism for vertebrate development research.
  • Efficient genomic analysis tools are crucial for fully exploiting the zebrafish system.
  • Gene mapping is essential for understanding genome organization and function.

Purpose of the Study:

  • To evaluate the utility of single-strand conformation polymorphism analysis (SSCP) for gene mapping in zebrafish.
  • To determine the polymorphism frequency of SSCP markers in zebrafish strains used for genetic mapping.
  • To assess the efficiency of SSCP in mapping transcribed sequences within the zebrafish genome.

Main Methods:

  • Analysis of over 100 primer pairs derived from noncoding gene regions and cDNAs.

Related Experiment Videos

  • Application of single-strand conformation polymorphism analysis (SSCP) to detect polymorphisms.
  • Localization of polymorphic cDNAs on the zebrafish genetic map.

    • Main Results:

    • A polymorphism frequency of approximately 50% was detected using SSCP in zebrafish strains.
    • SSCP proved to be an efficient strategy for high-resolution mapping of transcribed sequences.
    • A subset of polymorphic cDNAs was successfully localized on the zebrafish map.

    Conclusions:

    • SSCP is an effective and efficient method for gene mapping in zebrafish.
    • This technique facilitates the integration of existing zebrafish genetic maps and the creation of an EST map.
    • SSCP supports alternative gene localization strategies like comparative mapping, advancing zebrafish genomics research.