Effect of glucose on glycine requirement of Acanthamoeba castellanii

Acanthamoeba castellanii grows in a minimal medium (AMLIV) containing only arginine, methionine, leucine, isoleucine, and valine as sole nitrogen sources, other than vitamins, when glucose is the carbon source. With acetate as the carbon source, glycine must be added to AMLIV. Doubling time in AMLIV varies according to the ratio of amino acids concentrations. Several combinations yield Td values of approximately 70 hr.