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Fibronectin expression in bronchopulmonary dysplasia

R A Sinkin1, M Roberts, M B LoMonaco

  • 1Department of Pediatrics (Neonatology), Children's Hospital at Strong, 601 Elmwood Avenue, Box 651, Rochester, NY 14642, USA.

Pediatric and Developmental Pathology : the Official Journal of the Society for Pediatric Pathology and the Paediatric Pathology Society
|December 16, 1998
PubMed
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Fibronectin (FN) is elevated in neonatal Bronchopulmonary dysplasia (BPD) lungs. Pulmonary cells, not airway epithelium, synthesize FN, with levels peaking during BPD

Area of Science:

  • Neonatal lung disease
  • Extracellular matrix biology
  • Fibrosis research

Background:

  • Bronchopulmonary dysplasia (BPD) is a chronic neonatal lung disease.
  • Fibronectin (FN), an extracellular matrix protein, is elevated in BPD.
  • Pulmonary FN originates from plasma and local synthesis.

Purpose of the Study:

  • Identify pulmonary cells synthesizing FN.
  • Quantify FN abundance in BPD lungs.
  • Correlate FN levels with BPD stages.

Main Methods:

  • In situ hybridization for FN mRNA.
  • Immunohistochemistry for FN protein.
  • Analysis of neonatal autopsy lung specimens (BPD vs. controls).

Main Results:

Related Experiment Videos

  • FN mRNA and protein found in vascular endothelium, macrophages, fibroblasts, smooth muscle, and chondrocytes.
  • Hyaline membranes showed intense FN protein staining.
  • FN levels increased in early BPD, peaking in the chronic stage, and decreased in healed BPD.

Conclusions:

  • Pulmonary cells, excluding airway epithelium, synthesize FN.
  • Increased FN is associated with BPD progression and fibrotic changes.
  • Pulmonary cell-derived FN plays a role in BPD pathogenesis.