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Structural proteins of La Crosse virus

J F Obijeski, D H Bishop, F A Murphy

    Journal of Virology
    |September 1, 1976
    PubMed
    Summary

    La Crosse virus has three main proteins: two surface glycoproteins (G1, G2) and a nucleocapsid protein (N). This California encephalitis group virus is enveloped with surface projections.

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    Area of Science:

    • Virology
    • Molecular Biology
    • Microbiology

    Background:

    • La Crosse virus belongs to the California encephalitis group of bunyaviruses.
    • Understanding viral structure is crucial for developing antiviral strategies.

    Purpose of the Study:

    • To characterize the protein composition and structure of La Crosse virus.
    • To identify and quantify major virion proteins and describe particle morphology.

    Main Methods:

    • Purification of La Crosse virus particles.
    • Analysis of viral proteins using SDS-PAGE to determine molecular weights.
    • Isolation of nucleocapsids using nonionic detergent and salt treatment.
    • Electron microscopy for morphological analysis.

    Main Results:

    • Identified three major virion proteins: G1 (120 kDa), G2 (34 kDa), and N (23 kDa).
    • G1 and G2 are glycosylated surface glycoproteins present in equimolar amounts.
    • Nucleocapsids contain the N protein; a minor L protein (180 kDa) was also detected.
    • Electron microscopy revealed an enveloped virus particle (91 nm diameter) with surface projections (10 nm).

    Conclusions:

    • La Crosse virus possesses a defined protein composition essential for its structure and function.
    • The characterization provides a foundation for further research into bunyavirus replication and pathogenesis.

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