A novel DNA-binding motif in MarA: the first structure for an AraC family transcriptional activator
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Updated: Oct 28, 2026

CD Spectroscopy to Study DNA-Protein Interactions
Published on: February 10, 2022
S Rhee1, R G Martin, J L Rosner
1Laboratory of Molecular Biology, National Institutes of Diabetes, Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892-0560, USA.
Abstract:
A crystal structure for a member of the AraC prokaryotic transcriptional activator family, MarA, in complex with its cognate DNA-binding site is described. MarA consists of two similar subdomains, each containing a helix-turn-helix DNA-binding motif. The two recognition helices of the motifs are inserted into adjacent major groove segments on the same face of the DNA but are separated by only 27 A thereby bending the DNA by approximately 35 degrees. Extensive interactions between the recognition helices and the DNA major groove provide the sequence specificity.
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