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Related Experiment Videos

A locus control region regulates yeast recombination

J E Haber1

  • 1Rosenstiel Center, Keck Institute for Cellular Visualization, Brandeis University, Waltham, MA 02254, USA. haber@hydra.rose.brandeis.edu

Trends in Genetics : TIG
|September 2, 1998
PubMed
Summary

Yeast mating type switching involves replacing DNA at the mating-type (MAT) locus with copies from donor sites. Donor preference in Saccharomyces cerevisiae is controlled by a recombination enhancer.

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Area of Science:

  • Molecular Biology
  • Genetics
  • Yeast Biology

Background:

  • Yeast Saccharomyces cerevisiae switches mating types through DNA recombination at the mating-type (MAT) locus.
  • This process involves replacing 'a' or 'alpha' sequences with copies from HML or HMR donor loci on chromosome III.
  • Donor preference dictates whether MATa cells choose HML or MATalpha cells choose HMR.

Purpose of the Study:

  • To investigate the regulatory mechanisms controlling donor preference during yeast mating type switching.
  • To understand how the recombination enhancer influences the choice between HML and HMR donors.
  • To elucidate the locus control region's role in activating and deactivating recombination.

Main Methods:

  • Analysis of DNA recombination events in Saccharomyces cerevisiae.
  • Studies on the function of the 250 bp recombination enhancer.
  • Investigation of locus control region regulation.

Main Results:

  • MATa cells preferentially recombine with HML, while MATalpha cells preferentially recombine with HMR.
  • A 250 bp recombination enhancer regulates donor preference.
  • The locus control region's activity determines the on/off state of recombination.

Conclusions:

  • Donor preference in yeast mating type switching is a tightly regulated process.
  • The recombination enhancer and locus control region are key elements in controlling mating type switching.
  • Understanding these mechanisms provides insight into programmed DNA recombination.

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