Immunohistochemical detection for Actinomyces sp. in swine tonsillar abscess and granulomatous mastitis

The tonsils of eleven pigs and the mammary glands of a sow were used to investigate actinomycotic lesions due to Actinomyces sp. infection. At necropsy, there was no abnormality on these tonsils, on the other hand, numerous abscesses containing sulfur granules were found in the mammary. Histopathologically, the Actinomyces sp. lesions were noted as crypt abscesses in the tonsils and as pus-forming granulomas in the mammary glands. The microorganisms in both lesions were composed of bead-like cocci, bacillary cells and short, branching filaments, those cells being positive by the Gram's and Grocott's methods. Clubs were formed around the microbial clumps in these lesions. Immunohistochemically, there were cross-reactions between antibodies of Actinomyces sp. Chiba 101 (101) and swine actinomycetes of 7 species: A. bovis, A. hyovaginalis, A. israeli, A. naeslundii, A. pyogenes, A. suis) formerly Eubacterium suits) and A. viscosus. However it was possible to differentiate Actinomyces sp. 101 from them by absorption and dilution of the antiserum, then the microorganisms in the tonsillar crypt abscesses and the granulomatous mastitis were labelled with an immunoperoxidase technique using the absorbed Actinomyces sp. 101 antiserum. Thus, these immunolabelling properties are suggestive of the presence of 'A. suis' (Grässer) Franke 1973.