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Updated: Jul 26, 2026

Sheathless Capillary Electrophoresis–Mass Spectrometry for Metabolic Profiling of Biological Samples
Published on: October 1, 2016
Advances in capillary electrochromatography
M M Dittmann1, G P Rozing, G Ross
1Hewlett-Packard GmbH, Waldbronn Analytical Division, Germany.
Abstract:
This paper presents practical aspects of capillary electrochromatography (CEC). Preparation of capillary columns, including terminating frits and immobilization of the packed bed, is described in detail. Longevity and reproducibility of CEC columns is demonstrated. Instrumental aspects, sample introduction, sensitivity of detection, and gradient elution are discussed in detail. A number of examples that illustrate the versatility of CEC, in particular, for providing an isocratic separation alternative to conventional gradient separation in HPLC, are given.
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