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Ultrastructural changes in rabbit ciliary body after extraocular mitomycin C

R S Heaps1, J R Nordlund, F Gonzalez-Fernandez

  • 1University of Virginia, Department of Ophthalmology, Charlottesville 22906-0009, USA.

Investigative Ophthalmology & Visual Science
|September 4, 1998
PubMed
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Subconjunctival mitomycin C injections caused dose-dependent damage to rabbit ciliary body epithelium. Higher doses thinned cells and disrupted membranes, while lower doses showed minimal effects.

Area of Science:

  • Ophthalmology
  • Cell Biology
  • Toxicology

Background:

  • Mitomycin C is used in ophthalmic surgery.
  • Its effects on ocular tissues require detailed investigation.
  • Understanding ciliary body epithelium response is crucial.

Purpose of the Study:

  • To investigate ultrastructural changes in rabbit ciliary body epithelium.
  • To assess the impact of subconjunctival mitomycin C injections.

Main Methods:

  • Subconjunctival injections of varying mitomycin C doses in rabbit eyes.
  • Control eyes received balanced salt solution.
  • Electron microscopy analyzed ciliary body epithelium at injection and contralateral sites.

Main Results:

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  • Doses of 0.1 mg and higher caused epithelial thinning, vacuoles, and intercellular space expansion.
  • Apical membrane thinning and disrupted plasma membrane infoldings were observed.
  • Lower doses and controls showed normal ciliary body epithelium.

Conclusions:

  • Subconjunctival mitomycin C induces dose-dependent, localized ultrastructural alterations.
  • The ciliary body epithelium is sensitive to mitomycin C concentration.
  • These findings inform clinical use and risk assessment.