Related Experiment Videos
In vitro early allogeneic reaction of murine brain cortex cells
H Kovárů1, H Kozáková, Z Fisar
1First Medical Faculty, Charles University, Prague, Czech Republic.
Physiological Research
|January 1, 1997
Summary
Alloantigen exposure in brain cortex cells activates Na+,K+-ATPase and alters membrane fluidity. A peptide fraction (FA) mimics these allorecognition events, suggesting early regulatory peptide involvement in brain cell interactions.
Area of Science:
- Neuroscience
- Immunology
- Cell Biology
Background:
- Allogeneic reactions in brain cortex cells involve H-2 alloantigen-induced metabolic uncoupling.
- Early cellular responses to alloantigens are not fully understood.
Purpose of the Study:
- To investigate the early effects of alloantigen on brain cortex cell surface enzyme activity and membrane properties.
- To identify and characterize signaling molecules involved in allorecognition.
Main Methods:
- Measurement of Na+,K+-ATPase and Mg2+-ATPase activity.
- Analysis of cell membrane lipid fluidity.
- Isolation and functional analysis of peptide fractions from the supernatant.
Main Results:
- Alloantigen exposure increased Na+,K+-ATPase activity and membrane lipid fluidity.
- A peptide fraction (FA) enhanced Na+,K+-ATPase activity and blocked K+-evoked O2 uptake.
- Mg2+-ATPase activity remained unaffected.
Conclusions:
- Early allorecognition in brain cortex cells involves modulation of Na+,K+-ATPase and membrane properties.
- A cytokine-like peptide fraction plays a role in early allorecognition events.
- Brain cell allorecognition targets functionally important metabolic reactions.