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Genotypic and phenotypic characterization of Cryptosporidium parvum isolates from people with AIDS

G Widmer1, S Tzipori, C J Fichtenbaum

  • 1Division of Infectious Diseases, Tufts University School of Veterinary Medicine, North Grafton, Massachusetts 01536, USA.

The Journal of Infectious Diseases
|September 5, 1998
PubMed

Insights

Cryptosporidium parvum exhibits genetic subgroups, with human-associated (H) genotypes showing reduced virulence and infectivity in animal models compared to calf-associated (C) genotypes.

Area of Science:

  • Parasitology
  • Molecular Biology
  • Infectious Diseases

Background:

  • Cryptosporidium parvum shows genetic subgroups, but clinical relevance remains unclear.
  • Previous studies indicate heterogeneity in biochemical and antigenic characteristics.

Purpose of the Study:

  • To investigate the genotypes and virulence of Cryptosporidium parvum isolates from AIDS patients.
  • To correlate genotypic subgroups with infectivity in cell culture and animal models.

Main Methods:

  • Genotypic analysis of Cryptosporidium parvum oocysts from human immunodeficiency virus (HIV)-infected individuals.
  • Virulence assessment in cell monolayers and newborn ICR mice.
  • Comparison with a reference isolate (GCH1).

Main Results:

  • Ten of 13 isolates were human-associated (H) genotype; 3 were calf-associated (C) genotype.
  • H isolates exhibited lower virulence in cell culture compared to the reference isolate.
  • H isolates did not infect newborn mice, while one C isolate did.

Conclusions:

  • Cryptosporidium parvum genetic subgroups correlate with infectivity.
  • Further heterogeneity exists within these subgroups, impacting disease potential.

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