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Potential DNA mixtures introduced through kissing

S Banaschak1, K Möller, H Pfeiffer

  • 1Institute of Legal Medicine, Westfälische Wilhelms-Universität, Münster, Germany.

International Journal of Legal Medicine
|September 5, 1998
PubMed
Summary

Saliva DNA testing offers a safe alternative to blood, with cotton swabs being convenient. DNA mixtures from kissing were detected but only within 60 seconds of the first sample.

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Area of Science:

  • Forensic Science
  • Genetics
  • Biochemistry

Background:

  • DNA investigations often require numerous control samples.
  • Blood samples can be invasive and difficult to collect in large quantities.
  • Saliva offers a non-invasive alternative for DNA sample collection.

Purpose of the Study:

  • To compare DNA content across different saliva sampling methods: cotton wool swabs, filter paper, and liquid saliva.
  • To investigate the potential for DNA mixture detection in saliva samples following close contact, such as kissing.
  • To assess the reliability of saliva as a source for DNA analysis in forensic contexts.

Main Methods:

  • Collected saliva samples using three distinct techniques: cotton wool swabs, filter paper, and direct liquid collection.
  • Quantified DNA content from each sampling method.
  • Obtained saliva samples from consenting pairs after intensive kissing to analyze for mixed DNA profiles.
  • Utilized Short Tandem Repeat (STR) profiling to identify DNA patterns.

Main Results:

  • Cotton wool swabs provide a convenient and safe method for saliva DNA collection.
  • DNA content varied among the three sampling techniques.
  • Mixed STR patterns were identified in five samples collected after kissing.
  • These mixed DNA profiles were exclusively found in the initial sample taken within 60 seconds post-kissing.

Conclusions:

  • Saliva is a viable alternative to blood for DNA investigations, particularly for large control sample sets.
  • Cotton wool swabs are recommended as a practical and safe method for saliva DNA collection.
  • DNA transfer from kissing is detectable but transient, limited to immediate post-contact samples, suggesting careful timing is crucial in forensic sample collection.

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