Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Ion-channel-forming colicins

R M Stroud1, K Reiling, M Wiener

  • 1Department of Biochemistry and Biophysics, University of California, San Francisco School of Medicine 94143-0448, USA. stroud@msg.ucsf.edu

Current Opinion in Structural Biology
|September 8, 1998
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Enhancing the quality and reproducibility of research: Preferred Evaluation of Cognitive and Neuropsychological Studies - The PECANS statement for human studies.

Behavior research methods·2025
Same author

The contribution of the supplementary motor area to explicit and implicit timing: A high-definition transcranial Random Noise Stimulation (HD-tRNS) study.

Behavioural brain research·2023
Same author

Incomplete excision of basal cell carcinoma: combining multidisciplinary data gives a better overall understanding of risk.

Clinical and experimental dermatology·2021
Same author

Effects of exposure to ozone at different stages in the development of Plantago major L. on chlorophyll fluorescence and gas exchange.

The New phytologist·2021
Same author

High-pressure, high-temperature molecular doping of nanodiamond.

Science advances·2019
Same author

The nature, origin and modification of insoluble organic matter in chondrites, the possibly interstellar source of Earth's C and N.

Chemie der Erde : Beitrage zur chemischen Mineralogie, Petrographie und Geologie·2019
Same journal

Progress toward linking single-molecule behavior and condensate material properties.

Current opinion in structural biology·2026
Same journal

Tomogram exploration through template matching and deep learning.

Current opinion in structural biology·2026
Same journal

A comparative review of cryo-electron ptychography: Biological applications and future perspectives.

Current opinion in structural biology·2026
Same journal

Metabolic disruptions through a three-dimensional genomic lens.

Current opinion in structural biology·2026
Same journal

Collective variable design for biomolecular conformational dynamics.

Current opinion in structural biology·2026
Same journal

Polymer scaling in protein crowding: From dilute coils to semidilute meshes.

Current opinion in structural biology·2026
See all related articles

Recent studies reveal key features of ion-channel-forming colicins, including their structure, binding mechanisms to outer membrane porins, and translocation processes. These insights detail the formation of a specific transmembrane channel.

Area of Science:

  • Microbiology
  • Structural Biology
  • Biophysics

Background:

  • Colicins are bacteriocins, toxic proteins produced by bacteria.
  • Ion-channel-forming colicins are a class of these proteins that create pores in bacterial membranes.
  • Understanding their function is crucial for developing new antimicrobial strategies.

Purpose of the Study:

  • To elucidate the structural and mechanistic details of ion-channel-forming colicins.
  • To describe the process of colicin binding to outer membrane porins.
  • To characterize the translocation mechanism and the resulting transmembrane channel.

Main Methods:

  • Structural analysis of colicin domains.
  • Thermodynamic studies of protein-ligand interactions.

Related Experiment Videos

  • Biophysical characterization of channel formation.

    • Main Results:

    • Detailed four-domain structure of colicins identified.
    • Mechanism and thermodynamics of binding to outer membrane porin gating loops elucidated.
    • Translocation mechanism and competition for periplasmic transport pathways clarified.
    • Formation of a well-characterized, waisted, funnel-shaped transmembrane channel confirmed.

    Conclusions:

    • Recent advances provide a comprehensive understanding of ion-channel-forming colicin function.
    • These colicins utilize specific structural features for membrane insertion and pore formation.
    • Further research can leverage these findings for therapeutic applications.