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Self-amplification of satellite DNA in vitro
1Institute of Infectious Diseases and Immunology, Utrecht University, The Netherlands.
Genome
|September 8, 1998
Abstract:
We describe a PCR-like reaction in which genomic DNA acts as a template as well as a primer. Interaction between genomic tandem repeat units leads to self-amplification of satellite DNA. This genomic self-priming PCR (GSP-PCR) allowed the rapid amplification of species-specific tandem repeats of horse, cattle, dolphin, and chicken. A novel specific satellite of ostrich with a repeat unit of 60 bp was isolated using this method.