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Assignment of the mouse and cow CXC chemokine genes
W S Modi1, M R Amarante, M Hanson
1SAIC Frederick, National Cancer Institute-Frederick Cancer Research and Development Center, Frederick, MD, USA. modi@mail.ncifcrf.gov
Abstract:
Gene specific PCR primers were constructed for five mouse and three bovine CXC chemokine genes. The mouse genes were assigned using SSCP analyses of the Jackson BSS backcross panel to two groups on chromosome 5. One group containing Gro1 and Mip2 cosegregated with reference markers Alb1 and Btc, and was positioned 2.2 cM proximal to a group comprising Ifi10, Mig, and Scyb5. The bovine genes IL8, GRO1, and GRO3, mapped using bovine x hamster somatic cell hybrids, were all found to be located on chromosome 6. The locations of these genes in these two animal species are consistent with the positions in humans (4q13-->q21), and previous syntenic relationships among these three mammals.