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Related Experiment Videos

Tubule function in transgenic mice

T Wang1, G Giebisch

  • 1Department of Cellular and Molecular Physiology, Yale University School of Medicine, New Haven, CT 06520-8026, USA. Tong.Wang@yale.edu

Experimental Nephrology
|September 8, 1998
PubMed
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Genetically modified mouse models allow researchers to study kidney electrolyte transport by examining the absence of specific transporters. These models help understand how the kidney adapts to transport defects.

Area of Science:

  • Nephrology
  • Molecular Biology
  • Physiology

Background:

  • Numerous renal electrolyte transporters have been identified and cloned.
  • Genetically manipulated mouse models (transgenic knockout, overexpression) are crucial for studying ion transport in kidney tubules.
  • These models enable the investigation of kidney adaptive mechanisms to specific transport defects.

Purpose of the Study:

  • To review the physiological characterization of renal tubule transport in genetically modified animals.
  • To highlight the utility of mouse models with well-defined genetic transport lesions.
  • To explore kidney compensatory mechanisms in response to transporter disruptions.

Main Methods:

  • Utilizing genetically manipulated mouse models, including knockout and overexpression strains.

Related Experiment Videos

  • Focusing on models with targeted gene disruption (e.g., NHE2, NHE3, TSC, CFTR, H,K-ATPase, NHE1).
  • Physiological characterization of renal tubule transport in these defined genetic models.

    • Main Results:

    • Mouse models with disruptions in key transporters like NHE2, NHE3, TSC, CFTR, and H,K-ATPase have been developed.
    • Studies in these models reveal insights into the specific roles of transporters in renal electrolyte balance.
    • The kidney's adaptive responses to genetic transport lesions can be effectively studied.

    Conclusions:

    • Genetically modified mouse models are invaluable tools for dissecting renal electrolyte transport mechanisms.
    • These models facilitate the understanding of kidney physiology and adaptive responses.
    • Future research will benefit from the availability of additional transporter knockout models.