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HLA-B27 does not affect invasion of arthritogenic bacteria into human cells
O Ortiz-Alvarez1, D T Yu, R E Petty
1Department of Pediatrics, University of British Columbia, Vancouver, Canada.
Objective:
To investigate the effect of HLA-B27 expression on entry of Salmonella typhimurium and Yersinia enterocolitica into human cells.
Methods:
We performed standard bacterial invasion assays with S. typhimurium and Y enterocolitica to analyze isogenic pairs of HeLa (epithelial), U937 (promonocyte), C1R (B lymphocyte), and Jurkat (T lymphocyte) human cell lines and their respective HLA-B27 transfectants. Invasion of peripheral blood derived T lymphocytes, monocytes, and B lymphocytes/dendritic cell fraction (corresponding to peripheral blood cells depleted of monocytes and T lymphocytes) from patients with ankylosing spondylitis and healthy donors was also analyzed. The percentage of internalized bacteria was quantified, and the differences between HLA-B27 positive and negative samples were compared.
Results:
The percentages of intracellular S. typhimurium and Y enterocolitica in HeLa, U937, and C1R with or without B27 were not statistically different (independent t test). We also found that the percentage of internalized bacteria did not differ significantly between HLA-B27 positive and negative samples in the different populations of peripheral blood derived cells.
Conclusion:
The presence of HLA-B27 on the surface of human cells does not alter the degree of bacterial invasion into either cultured human cell lines or peripheral blood derived human cells, and the influence of HLA-B27 expression on bacterial invasion should not be implicated in the pathogenesis of reactive arthritis related to Salmonella and Yersinia.
Insights
Human leukocyte antigen B27 (HLA-B27) expression does not affect the invasion of Salmonella typhimurium or Yersinia enterocolitica into human cells. This finding suggests HLA-B27 is not involved in reactive arthritis pathogenesis related to these bacteria.
Area of Science:
- Immunogenetics
- Microbiology
- Cell Biology
Background:
- Human leukocyte antigen B27 (HLA-B27) is associated with autoimmune diseases like reactive arthritis.
- Reactive arthritis can be triggered by bacterial infections, including Salmonella and Yersinia.
Purpose of the Study:
- To determine if HLA-B27 expression influences the entry of Salmonella typhimurium and Yersinia enterocolitica into human cells.
- To investigate the role of HLA-B27 in the pathogenesis of reactive arthritis.
Main Methods:
- Standard bacterial invasion assays were conducted using various human cell lines (HeLa, U937, C1R, Jurkat) and their HLA-B27 transfectants.
- Invasion assays were also performed on peripheral blood cells (T lymphocytes, monocytes, B lymphocytes/dendritic cells) from healthy donors and patients with ankylosing spondylitis.
- The percentage of internalized bacteria was quantified and compared between HLA-B27 positive and negative cells.
Main Results:
- No statistically significant difference in the percentage of intracellular Salmonella typhimurium and Yersinia enterocolitica was observed between HLA-B27 positive and negative cells in cultured cell lines.
- Similarly, bacterial internalization rates did not differ significantly between HLA-B27 positive and negative peripheral blood derived cells.
Conclusions:
- The presence of HLA-B27 on human cells does not alter bacterial invasion.
- HLA-B27 expression is unlikely to be a significant factor in the pathogenesis of reactive arthritis caused by Salmonella and Yersinia infections.