Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Intermolecular Forces03:13

Intermolecular Forces

62.9K
Atoms and molecules interact through bonds (or forces): intramolecular and intermolecular. The forces are electrostatic as they arise from interactions (attractive or repulsive) between charged species (permanent, partial, or temporary charges) and exist with varying strengths between ions, polar, nonpolar, and neutral molecules. The different types of intermolecular forces are ion–dipole, dipole–dipole, hydrogen bonds, and dispersion; among these, dipole–dipole, hydrogen...
62.9K
Protein-protein Interfaces02:04

Protein-protein Interfaces

12.5K
Many proteins form complexes to carry out their functions, making protein-protein interactions (PPIs) essential for an organism's survival. Most PPIs are stabilized by numerous weak noncovalent chemical forces. The physical shape of the interfaces determines the way two proteins interact. Many globular proteins have closely-matching shapes on their surfaces, which form a large number of weak bonds. Additionally, many PPIs occur between two helices or between a surface cleft and a...
12.5K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

[Survey analysis on the development and current status of pediatric digestive endoscopy over past 40 years].

Zhonghua er ke za zhi = Chinese journal of pediatrics·2026
Same author

[Annual advances in chronic cough 2025].

Zhonghua jie he he hu xi za zhi = Zhonghua jiehe he huxi zazhi = Chinese journal of tuberculosis and respiratory diseases·2026
Same author

[Advances in the application of next generation sequencing for pathogenesis researches of adenomyosis].

Zhonghua fu chan ke za zhi·2026
Same author

[Milnacipran for the treatment of refractory chronic cough: a report of two cases].

Zhonghua jie he he hu xi za zhi = Zhonghua jiehe he huxi zazhi = Chinese journal of tuberculosis and respiratory diseases·2026
Same author

[Analysis on epidemiology trends of overweight, obesity, and body mass index in adults aged 18-69 years in Shandong Province, 2004-2023].

Zhonghua liu xing bing xue za zhi = Zhonghua liuxingbingxue zazhi·2025
Same author

[Bendamustine combined with anti-CD20 monoclonal antibody in the first-line treatment of older patients with indolent B-cell non-Hodgkin lymphoma: a multicenter retrospective study].

Zhonghua xue ye xue za zhi = Zhonghua xueyexue zazhi·2025
Same journal

Erratum for the Research Article "Detecting supramolecular organic nanoparticles during heat wave".

Science (New York, N.Y.)·2026
Same journal

Local signals, systemic decline.

Science (New York, N.Y.)·2026
Same journal

The mechanics of liver regeneration.

Science (New York, N.Y.)·2026
Same journal

Computing in a memory with physics.

Science (New York, N.Y.)·2026
Same journal

Retraction.

Science (New York, N.Y.)·2026
Same journal

Making time.

Science (New York, N.Y.)·2026
See all related articles

Related Experiment Video

Updated: Apr 30, 2026

A Microfluidic-based Hydrodynamic Trap for Single Particles
10:13

A Microfluidic-based Hydrodynamic Trap for Single Particles

Published on: January 22, 2011

16.0K

Long-range electrostatic trapping of single-protein molecules at a liquid-solid interface

X H Xu1, E S Yeung

  • 1Ames Laboratory-U.S. Department of Energy and Department of Chemistry, Iowa State University, Ames, IA 50011, USA.

Science (New York, N.Y.)
|September 11, 1998
PubMed
Summary
This summary is machine-generated.

Single protein molecule motion near surfaces reveals surface charge influences protein behavior beyond the electrical double layer. This study verifies statistical chromatography theory at the single-molecule level, highlighting long-range trapping over adsorption.

More Related Videos

Optical Trapping of Nanoparticles
13:39

Optical Trapping of Nanoparticles

Published on: January 15, 2013

27.3K
Author Spotlight: Evaluation of Protein-Condensate Dynamics in Live Human Cells
06:48

Author Spotlight: Evaluation of Protein-Condensate Dynamics in Live Human Cells

Published on: January 5, 2024

5.3K

Related Experiment Videos

Last Updated: Apr 30, 2026

A Microfluidic-based Hydrodynamic Trap for Single Particles
10:13

A Microfluidic-based Hydrodynamic Trap for Single Particles

Published on: January 22, 2011

16.0K
Optical Trapping of Nanoparticles
13:39

Optical Trapping of Nanoparticles

Published on: January 15, 2013

27.3K
Author Spotlight: Evaluation of Protein-Condensate Dynamics in Live Human Cells
06:48

Author Spotlight: Evaluation of Protein-Condensate Dynamics in Live Human Cells

Published on: January 5, 2024

5.3K

Area of Science:

  • Biophysics
  • Surface Science
  • Analytical Chemistry

Background:

  • Understanding protein behavior at interfaces is crucial for various applications.
  • The electrical double layer's influence on charged molecules is well-established but its range is debated.
  • Single-molecule techniques offer high resolution for studying interfacial phenomena.

Purpose of the Study:

  • To investigate the motion and interfacial behavior of single protein molecules.
  • To determine the influence of surface charge and solution conditions (pH, ionic strength) on protein-molecule interactions.
  • To validate statistical chromatography theory at the single-molecule level.

Main Methods:

  • Monitoring the motion of single, dye-labeled protein molecules.
  • Utilizing an evanescent-field layer at a fused-silica surface.
  • Varying pH and ionic strength to control surface charge and molecular charge.

Main Results:

  • Increased protein molecule partitioning into the excitation region below the isoelectric point.
  • Random spatial distribution of molecules, indicating surface charge influence beyond the electrical double layer.
  • Increased residence times in the interfacial layer below the isoelectric point, without permanent immobilization.
  • Residence time histograms mirrored elution peak asymmetry in capillary electrophoresis.

Conclusions:

  • Surface charge significantly impacts charged protein behavior at interfaces, extending beyond the predicted electrical double-layer thickness.
  • The observed phenomena align with statistical chromatography theory, with long-range trapping as the primary interaction mechanism.
  • Single-molecule analysis provides direct experimental verification of theoretical models in interfacial science.